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Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

These are major expansions that add new gameplay mechanics and story levels:
Review: LittleBigPlanet 2 Extras Edition (PlayStation 3) Released in as a Europe-exclusive retail package, LittleBigPlanet 2: Extras Edition is the definitive "Director’s Cut" of the critically acclaimed creative platformer. This version bundles the original game with a massive collection of premium DLC on a single disc, offering a significantly more complete experience than the standard release. What’s Included in the Extras Edition? Little Big Planet 2 Extras Edition
The Extras Edition was specifically designed to highlight Sony’s hardware integration, including support for the and PS Vita Cross-Controller features. Premium Level Kits These are major expansions that add new gameplay
: A unique pack that allows you to use a PS Vita as a secondary controller for exclusive puzzles. The Extras Edition was specifically designed to highlight
The edition also includes six specialized costume packs to customize Sackboy: Weekend Pursuits Costume Pack Deep Sea Adventures Costume Pack Sports Costume Pack Dogs Costume Pack Cats Costume Pack Extras Edition vs. Special Edition
: Adds 14 new levels (5 story, 7 mini-games, 2 cutscenes) centered on the "Brain Crane" power-up and PlayStation Move motion controls.
: Includes a Muppets-themed adventure and the "Great Gonzo" costume. DLC Costume Packs